Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA Viral RNA. [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2Article Published on 2022-12-012022-11-15 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome amplification amplify catalytic clinical sample coronavirus coronavirus disease COVID-19 develop Diagnosis gold standard highlighting HPV-16 limit of detection Nanozyme strips nontoxic nucleic acid nucleic acid amplification Nucleic acid detection pandemic POCT point-of-care point-of-care testing polymerase REcombinase recombinase polymerase amplification reliability RPA RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. subsequent TMB unique viral infections virus [DOI] 10.1016/j.bios.2022.114739 PMC 바로가기
Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveysArticle Published on 2022-12-012022-11-15 Journal: Diagnostic microbiology and infectious disease [Category] COVID19(2023년), SARS, 진단, [키워드] anti-spike protein IgG antibody approach Brazil change changes in Community-based cohort conducted decrease Dilution dilutions ELISA evaluate fitting IgG ELISA Immunity incidence increase interpolated MONITOR normalized optical optical density pandemic Participants predicted reduce reliability resource SARS-CoV-2 sera serial dilutions serology Serosurvey significantly titers Titers. was performed [DOI] 10.1016/j.diagmicrobio.2022.115807 PMC 바로가기
Attitude toward COVID-19 vaccines and its association with depressive symptoms in 386,924 Chinese primary school students during COVID-19 epidemic normalizationResearch article Published on 2022-11-012022-10-05 Journal: Journal of psychosomatic research [Category] 변종, [키워드] 95% confidence interval addition adjusted odds ratio Analysis association Attitude children China Chinese Cluster COVID-19 COVID-19 epidemic COVID-19 infection COVID-19 vaccine COVID-19 vaccines cross-sectional depressive symptom Depressive symptoms evaluate evaluated Factor Government information inventory involved lower risk multiple logistic regression Participants positive attitude Prevalence promote PROTECT reliability Result Safe School the vaccine vaccination Vaccine was used were used [DOI] 10.1016/j.jpsychores.2022.111021 [Article Type] Research article
Association of diaphragm thickness and density measured on chest CT with disease severity in COVID-19 patientsResearch article Published on 2022-11-012022-10-05 Journal: The American journal of emergency medicine [Category] SARS, 진단, [키워드] 95% confidence interval addition Admission age analyses analysis analyzed Bilateral celiac artery Chest computed tomography Chest CT Clinical course Clinical outcome Comorbidities Computed tomography coronavirus disease correlation COVID-19 COVID-19 patient decrease demographic characteristics demonstrated Diaphragm disease severity evaluate evaluated Hemidiaphragm ICU admission independent predictor independent risk factor investigated Logistic regression Mortality multivariable model muscle mass Odds ratio Patient patients with COVID-19 performed Pneumonia polymerase chain positive Prognosis Prognostic markers reliability Result risk factor severe disease statistically significant the patient threshold value was measured [DOI] 10.1016/j.ajem.2022.08.029 [Article Type] Research article
Systematic comparison of ranking aggregation methods for gene lists in experimental resultsArticle Published on 2022-10-312022-11-16 Journal: Bioinformatics [Category] COVID19(2023년), meta-analysis, SARS, [키워드] addition aggregation Algorithm Apoptosis automated Bacteria biological process Cancer Cell Characteristics code Consensus database dataset datasets disease downloaded Evaluating Features genomic data heterogeneity implementation implicated Inclusion information lack list of gene lung cancer Macrophage Meta-analysis noise level question reliability required resulting specific type Support the SARS-CoV-2 virus Variation was performed were used [DOI] 10.1093/bioinformatics/btac621 PMC 바로가기
COVID-19 Outbreak Forecasting Based on Vaccine Rates and Tweets ClassificationArticle Published on 2022-10-272022-11-16 Journal: Computational Intelligence and Neuroscience [Category] 치료기술, [키워드] affected Analysis applied caused classification Combination confirmed cases country COVID-19 disease early stage Effectiveness ENhance feature Forecasting glove Health healthcare help IMPROVE infected case introduced machine learning algorithm machine learning model outbreak public health Rate reliability resource spread of COVID-19 Surveillance SVM the disease Vaccine Vaccines virus spread [DOI] 10.1155/2022/4535541 PMC 바로가기
Ultrafast one-minute electronic detection of SARS-CoV-2 infection by 3CLpro enzymatic activity in untreated saliva samplesArticle Published on 2022-10-262022-11-15 Journal: Nature Communications [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] 3CLpro acute respiratory syndrome applicability approach Asymptomatic individuals carried caused clinical test coronavirus death detect enzymatic activity enzyme Infection marker PCR testing pH indicator platform point-of-care proteolytic proteolytic activity reliability Saliva SARS-CoV-2 SARS-COV-2 infection Sensitivity and specificity subject Transmission Viral transmission [DOI] 10.1038/s41467-022-34074-2 PMC 바로가기
Rapid Identification of Drug Resistance and Phylogeny in M. tuberculosis, Directly from Sputum SamplesArticle Published on 2022-10-262022-11-15 Journal: Microbiology Spectrum [Category] 바이오마커, 변종, 치료제, [키워드] Accuracy applied approaches clinical sample clinician Complete COVID-19 pandemic Culture deaths described detect Diagnosis diagnostic test disease drug drug resistance drug susceptibility drugs effective Establishing evaluate evaluated faster genetic marker Genetic variant genetics genomic variant helping identification identify infecting Infectious disease information initial Initially molecular molecular test Mutation Mycobacterium Mycobacterium tuberculosis negatively affected Next-generation sequencing NGS phenotypic Phylogenetic phylogenetically Phylogeny protocol Rapid reducing reliability reported resistance responsible resulting Sample sequenced Sequencing sputum Sputum sample sputum samples Stage strain targeted sequencing TB patient the WHO Treatment Tuberculosis tuberculosis. was used WGS WHO [DOI] 10.1128/spectrum.01252-22 PMC 바로가기